species diversity of indigenous trichoderma from alkaline pistachio soils in iran

in the present study, the diversity of trichoderma spp. was investigated in alkaline pistachio soils at different geographic area in kerman province, iran. a total of 161 trichoderma isolates were obtained and identified at the species level by analysis of their morphological characters and sequence analysis of their internal transcribed spacer regions of the rrna cluster and tef1 genes as phylogenetic markers. based on morphological characteristics and sequence analysis five species of trichoderma including t. harzianum (87 isolates), t. aureoviride (44 isolates), t. virens (19 isolates), t. brevicompactum (9 isolates) and t. longibrachiatum (2 isolates) were isolated and identified. the complete its regions nucleotide sequences of the rrna gene of 17 selected isolates of t. harzianum and t. aureoviride and 4 selected isolates of t. virens, t. brevicompactum and t. longibrachiatum were determined. our data showed that very little its sequence variation was observed and did not find molecular differences between t. harzianum and t. aureoviride. these species were not able to separate based on rrna its region. to identify and differentiate these two species, the sequences fragment of the translation-elongation factor 1- (tef1-int4 (large)) gene were determined for 5 isolates of t. harzianum and t. aureoviride. the blast similarity search using the sequences of the isolates determined in this study for t. harzianum and t. aureoviride revealed as the h. lixii. however, there were 34 nucleotide differences between the two groups of isolates.